MB041-EN-1: dNTP Mix, 10 mM each
Chemically synthesized and >99% purity confirmed by HPLC;
Free of human and E. coli DNA;
Free of DNases and RNases;
Free from PCR, qPCR, and cDNA synthesis inhibitors;
Stable for 2 years at –20°C.MB066-EN-1: dNTP mix, 25 mM each
MB066-EN-2: dNTP set, 100 mM each
Chemically synthesized and >99% purity confirmed by HPLC;
Free of human and E. coli DNA;
Free of DNases and RNases;
Free from PCR, qPCR, and cDNA synthesis inhibitors;
Stable for 2 years at –20°C.
MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
* Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
* Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
* Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
* Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.MB016-HYT: High Yield Taq DNA Polymerase
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.MB118: Hot-Start High-Fidelity DNA Polymerase
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Fast amplification speed: normally ~30 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional Taq DNA polymerase.
* High specificity: Its amplification mismatch rate is 1/53 that of ordinary Taq polymerase and 1/6 that of Pfu polymerase.
* Reproducible results: even with the GC-rich fragments and in the presence of PCR inhibitors.
* Blunt-ended amplified products.
* Low cost: $0.8 per unit. The cheapest hot start high fidelity Taq enzyme in the market.MB040: GC rich PCR Enhancer
The GC rich PCR enhancer can improve the efficiency of PCR amplification of GC rich DNA templates, increase the specificity of PCR products, and reduce non-specific and undesirable PCR products.MB049-EQ2G: 2x Rapid PCR Master Mix, with green dye
* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
* Fast amplification speed: normally ~15 sec/kb; ~1 sec/kb if the amplification length is <1 kb.
* High stability: repeated freezing and thawing for 50 times without significant decrease in activity.
* Low cost: $400 for 50 ml 2x rapid PCR PreMix with dye. The cheapest PCR master mix for genotyping in the market.MB042-EUT: Taq DNA Polymerase
* High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
* Low cost: 2.8+ cents per unit. The cheapest PCR enzyme in the market.